Composite

Part:BBa_K2749003:Design

Designed by: iGEM 2018 Ruia-Mumbai Team   Group: iGEM18_Ruia-Mumbai   (2018-10-10)


pBAD_ompT_xylE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 891
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is ensured that there are no EcoR1 and Pst1 restriction sites in between the sequence for the final construct. The sequences were codon optimized for the chassis E. coli. Double stop codons are added after the xylE_histag(6x) basic part for the final sequence of the construct.



Source

xylE is derived form genomic sequence of Pseudomonas putida ompT is derived from genomic sequence of Escherichia coli

References